Regulatory

Part:BBa_K4129001:Design

Designed by: Giovanni Carlo Gendarini   Group: iGEM22_DTU-Denmark   (2022-10-02)


Promoter of the Saccharomyces cerevisiae FDH2 gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 275
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was designed to be used with Golden Gate assembly. The genomic sequence did not conatin any BsaI site, therefore no modifications were made.


Source

The part was amplified by PCR from gDNA

References